David Kesler wrote:

>I would like to perform a laboratory exercise demonstrating the
>antibiotic properties of propolis.  Most simply this would be measuring
>the halo around propolis tablets on a bacterial plate.   If you have any
>suggestions, such as bacterial strain, etc.  they will be most welcomed.
>
Depends on how the prep will be used.  As a mouthwash?  They I would hit
up the local hospitals micro lab for common strains found in people with
oral lesions. (A dental school would be even better).  A a part of a
dressing for wounds, perhaps a burn unit etc.  It all depends on how you
will use it later.  Not all bacteria are the same, nor are they treated
with the same materials and delivery so it is very very dependant on the
execution you have in mind.  Simply measuring a halo on  a plate around
a disk is, well, not going to tell you a whole heck of a lot.

Get in touch with a clinical microbiologist - there are far better ways
to skin this cat than using disk diffusion methodologies - of course
even that depends on your final clinical application.

Keith

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