Hi Georges
Thanks for the insight... I am pretty familiar with Nosema spores. I've been crushing bees for a few years now. I am also in the process of building my pollen ref library. I checked and we don't what you indicated. I have some stain (Safranin-O and Fuchsin) coming soon that I will add to my PVA-G mounting solution. I hope the stain will help improve the contrast a bit more. I also found a few data tables with under/over/average representation factors.
Here is the tool I am currently building to help with my pollen counting and identifying. I am an engineer so collecting data in a very structured way is important for me. Remember I only have a few hours under my belt. Any advice to help develop my approach is appreciated. the 3 main pollens in the sample where lingonberry, Jacob's Ladder and willow (75% sure). The rest are not very numerous (fireweed, siberian asters.... and a bunch I haven't seen yet). I have a separate ref guide that I am building as I go. https://drive.google.com/file/d/1pozdq_xVVcFIn3DjJWdVdfIRWFnYJlxZ/view?usp=sharing
For the group: Are Nosema spores in honey still viable? Have any studies looked into honey being an infection vector? Consuming 50lbs of Nosema infected honey over a winter would likely potentially (theory) be one of the reasons why spring Nosema is worse in cold climate colonies, especially if there are no signs of dysentery (as a possible other vector).
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