>
> >I think the midgut of a 5th-instar larva can be teased out for
> examination but a procedure would need to be developed. I almost got a few
> out but using my crude methods the epithelial lining was too thin to hold
> the contents together so I defaulted to looking at a homogenized mass. The
> result is a much less concentrated mixture and the pollen cells were few
> and far between.


I've had the same difficulty.  So I tested to see whether looking at the
homogenized body contents would be sufficient by spiking some with pollen
grains from the guts of an adult bee.  It is easy to spot them in the
homogenate.


-- 
Randy Oliver
Grass Valley, CA
www.ScientificBeekeeping.com

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