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Tue, 15 Sep 2020 10:25:14 -0300
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Informed Discussion of Beekeeping Issues and Bee Biology <[log in to unmask]>
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worst than SArc-Cov-2?

https://www.researchgate.net/publication/336305301_Microsporidia_Nosema_spp_-_obligate_bee_parasites_are_transmitted_by_air

Abstract ..

Microsporidia Nosema are transferred among bees via the faecal-oral route.
Nosema spp. spores have been detected on flowers and transferred to hives
along with the bee pollen. The aim of the present study was to determine
whether Nosema microsporidia are transferred by air in an apiary, in a
control area (without the presence of bee colonies), and/or in a laboratory
during cage experiments with artificially infected bees. The novel way of
transmission by air was investigated by the volumetric method using a
Hirst-type aerobiological sampler located on the ground in the apiary, in
the Botanical Garden and on the laboratory floor. Concurrently, the mean
rate of Nosema infections in the foragers in the apiary was estimated with
the Bürker haemocytometer method. Spore-trapping tapes were imaged by means
of light microscopy, Nomarski interference contrast microscopy and scanning
electron microscopy. The highest concentration of Nosema spores per 1m3 of
air (4.65) was recorded in August, while the lowest concentration (2.89)
was noted in July. This was confirmed by a Real-Time PCR analysis. The
presence of N. apis as well as N. ceranae was detected in each of the
tested tapes from the apiary. The average copy number of N. apis was
estimated at 14.4 × 104 copies per 1 cm2 of the tape; whereas the number of
N. ceranae was 2.24 × 104 copies per tape per 1 cm2. The results indicate
that Nosema microsporidia were transferred by the wind in the apiary, but
not in the Botanical Garden and laboratory by air. This was confirmed by
genetic analyses. DNA from immobilised biological material was isolated and
subjected to a PCR to detect the Nosema species. A fragment of the 16S rRNA
gene, characteristic of Nosema apis and N. ceranae, was detected. Our
research adds knowledge about the transfer of Nosema spp.

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