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Subject:	Re: Problematic for the wider beekeeping community
From:	Bill Hesbach <[log in to unmask]>
Reply To:	Informed Discussion of Beekeeping Issues and Bee Biology <[log in to unmask]>
Date:	Sun, 14 Mar 2021 10:21:51 -0400
Content-Type:	text/plain
Parts/Attachments:	text/plain (22 lines)

> These often lack key controls - for example, demonstrating replication of DWV in "symptomatic" Bombus.

If you have the means to determine symptomology, wouldn't that be enough to verify viral replication? 


 >Our own work (Gusachenko 2020 Scientific Reports 10 16847) has demonstrated replication of DWV in Bombus - following direct inoculation of purified virus stocks and analysis of negative strand production - but with no evidence for any wing deformities. 

How did you determine that your results were measuring infectious particles? Also, did you look at the cycle threshold numbers?

> However, even after feeding Bombus colonies 10^8 DWV per day for a month, we were unable to detect any replicating virus in larvae, pupae or adults.

Is it possible that what you fed were not infectious particles. 


Bill Hesbach
Cheshire CT

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