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Subject:	Re: Nosema fluorescent stain
From:	Georges Prigent <[log in to unmask]>
Reply To:	Informed Discussion of Beekeeping Issues and Bee Biology <[log in to unmask]>
Date:	Thu, 7 Mar 2019 17:04:15 -0500
Content-Type:	text/plain
Parts/Attachments:	text/plain (13 lines)

If so, can you offer some help with a source for a stain that will work? 

Hello Bill,
This is because they are using a fluorescent microscope using UV light, in this case the die is for exemple calcofluor white https://www.sigmaaldrich.com/catalog/product/sial/18909?lang=fr&region=FR&gclid=EAIaIQobChMI3JyxsIHx4AIVElXTCh2YtgzmEAAYASAAEgKTXPD_BwE
But if you are using a standard microscope such as Randy explained us, you need instead to counterstain, so the spores appear transparent.

Georges

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