>Our findings showing that the honey bee egfr gene belongs to the non-methylated category make the result shown in the Kamakura paper impossible to reproduce. There are three lines of evidence questioning the correctness of the original claim. First, on the basis of its high CG content, egfr is bioinformatically predicted to be non-methylated. Second, genome-wide methylation profiles in larvae, brains and embryos from both queens and workers show no evidence of methylation.
EGFR gene methylation is not involved in Royalactin controlled phenotypic polymorphism in honey bees
R. Kucharski, S. Foret & R. Maleszka
https://www.nature.com/articles/srep14070
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