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Date: | Sat, 17 May 2014 07:47:01 -0400 |
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> We report the first to our knowledge genetically engineered honeybees, which are important pollinators and interesting biological models for the study of social and complex behaviors as well as caste and sexual development. This genetic manipulation tool will enable systematic studies of biological processes in an organism building complex societies. We demonstrate highly efficient integration and expression of piggyBac-derived cassettes in the honeybee that make this system applicable to colony-based screening approaches and useful for an average beekeeping facility. This cassette was stably and efficiently transmitted and expressed in progeny by two different promoters, offering the prospect for activation or inhibition of gene functions under conditions of stage- and tissue-specific promoters.
Schulte, C., Theilenberg, E., Müller-Borg, M., Gempe, T., & Beye, M. (2014). Highly efficient integration and expression of piggyBac-derived cassettes in the honeybee (Apis mellifera). Proceedings of the National Academy of Sciences,
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