>The emerging brood now appears spotty, and mites and DWV are visible, along
with demoralized adult bees.

I see similar, Jeremy, but then the nuc outbreeds the mite for a while.
The spotty brood is a major issue IMO, as it likely indicates virus issues.

In a perfect world, I would treat all nucs made with queen cells at Day 21,
the short window in which the old brood has all emerged, and few new cells
have been capped.  An oxalic dribble on that day works great, but it
requires better time organization than I am generally able to muster.

Randy Oliver

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