Medhat wrote
> When we tested thousands of colonies when I was in Ontario, we found the
> following to get reliable results you need to:
> 1. Pick a frame of capped brood that contains pupae with light pink eyes.
> 2. use a smaller ring that isolates 30-50 cells to be treated with Liquid
> nitrogen.
> 3. Use 4 replicates /hive that means 4 rings placed on a single capped
> brood frame Then apply your liquid nitrogen . The total cells treated is
> 120-200 capped cells in each hive.
I am interested to know if there was testing done to compare the results of
a single dose, as per the conventional way, to the 4 replicates that Medhat
used.
Trevor Weatherhead
AUSTRALIA
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