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Informed Discussion of Beekeeping Issues and Bee Biology

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Fri, 31 Oct 2008 10:30:43 EDT
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Informed Discussion of Beekeeping Issues and Bee Biology <[log in to unmask]>
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Jerry Bromenshenk <[log in to unmask]>
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Thanks to Jim for doing the math about ppb - now, two  warnings.  
 
Parts per billion is not a universally consistent term.  What we in  the U.S. 
define as PPB IS NOT what much of the rest of the English speaking  world 
call PPB.
 
Second, when reading ANY European dose response literature, you have to be  
very careful about a term called mg% sneaking into the reported results.  
 
One also sometimes sees this term in Medical literature.  That  throws 
another factor of 100 into the equation - and ITS A COMMON mistake made -  to not 
recognize this term for what it is and adjust the reported dose to the  true 
dose - especially when literature is translated from another  language into 
English.
 
Finally, much of the pesticide testing is done by feeding - and you have to  
consider whether each bee actually consumed the calculated amount.
 
However, some testing uses contact toxicity.  In the lab, this  consists of 
putting a small drop (microliter) containing the chemical onto the  body of 
each tested bee.  Tricky thing here is that the bees are  usually put to sleep to 
facilitate this - but many labs hit the bees too hard  with the anaesthesia 
OR use too much carrier chemical, inducing a high mortality  of controls.  The 
error is then compounded when the investigator fails to  adjust for control 
mortality in the final report.
 
Larry Atkins and I had long conversation about this.  If you do  it right, 
your control mortality should be less than 5%.  That is NOT  the case for many 
of the trials that I've reviewed.
 
Jerry
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