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Hi All,
I haven't used the liquid nitrogen procedure, what kind of DNA are you
extracting?  genomic or mitochondrial?  Genomic DNA will run as a smear and
I don't think you'll see any differences in banding patterns when cut.  I
used a specific DNA sequence that was radiactively labelled on a Southern
blot of genomic DNA (after cutting with different restriction enzymes) to
see distinguishing patterns.

Unless someone else has already done it, you'll have to look at the DNA
sequences itself and look for restriction sites.  You need to use a sequence
comparison tool -- CLUSTAL, or our Sequence Server -- pull up the sequences
from GenBank and then search for the restriction sites.  You could also cut
and paste the sequence into Word and then do a search for the restriction
sites.  I'm suggesting the former because you can line up all the sequences
at once and look specifically at places where they differ for restriction
sites.

If you provide more info, I may be able to help more.

Regards,
Shirley

**********************************************************
Shirley Chan, Ph.D.
Dolan DNA Learning Center
Multimedia Producer
One Bungtown Road
Cold Spring Harbor, NY 11724

V: 516 367 5179
F: 516 367 5182

Dolan DNA Learning Center -- http://www.dnalc.org
DNA from the Beginning -- http://www.dnaftb.org
Your Genes, Your Health -- http://www.yourgenesyourhealth.org
DNA Interactive -- http://www.dnai.org

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