David wrote:
I would like to perform a laboratory exercise demonstrating the
antibiotic properties of propolis.  Most simply this would be measuring
the halo around propolis tablets on a bacterial plate.   If you have any
suggestions, such as bacterial strain, etc.  they will be most welcomed.


Hi David and all,
I can not help too much, but that question is very interesting to me and I
would like to learn something more about that as you too.
Being editor of one collection of beekeeping articles I was in contact with
one young professor from an school of chemical engineering who had the
article about bacteridal properties of propolis.

he used microorganisms:
Escherichia coli 2592
Staphylococcus aureus 6538
Bacillus subtilis 6633
Proteus vulgaris
Pseudomonas aeruginosa 9027
Candida albicans 10231 (fungus)

Antibiotics:
G10 and G2.5 solution of gentamycin 10 IE/ml (2.5 IE/ml)
N100 and N50  neomycin 100 IE/ml (50 IE/ml)
C5 and C2.5 ciprofloxacin 5 g/ml (2.5 g/ml)

propolis extracts in 70% ethyl alcohol (nondissolved and dissolved 1:2,
1:10, 1:25)

He used diffusion method with cellulose disks. (?)

It is interesting that propolis showed stronger bactericidal properties in
comparison with antibiotics.

Regarding purchasing microorganisms and equipment I can recommend:

www.coleparmer.com
cat. numb. for Escherichia coli  A-14013-26 or A-14013-28 or A-14016-16 etc.

best regards
Predrag Cvetkovic, Serbia
[log in to unmask]

::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
-- Visit www.honeybeeworld.com/BEE-L for rules, FAQ and  other info ---
::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::