Dear Brad.
    You may have killed your "original queen" with your over zealous
inspections.
If you did, the likelihood is that you now have a "new" queen in your
hive.  Give it time to mate and to develop her ovaries.  Also, when your
bees start to bring in fresh nectar, if you have a mated queen, she'll
start to lay. Giving that hive a frame with one day old eggs will ensure
a replacement queen for you if in fact there is no queen in there now,
and you will have not done any harm.  If there is a queen, nothing will
happen.  Please make sure that you smoke that brood frame well before
you insert it into the new hive to dilute the pheromones of the queen
from which you are removing it. Just a good precaution.
Good luck and best regards.
Dr. Rodriguez