In a message dated 96-11-07 22:21:42 EST, you write: >She wants to know "What >is the worse thing that can happen if I use the twice frozen milk?" We >could find nothing on this, but offered to check the net for her. Does >anybody have info on this? Sorry, there is no info or references on this. I can only contribute info from my clinical experience. I have had several phone calls on this. I had to explain that the recommendations are to not refreeze breast milk. We speculate that more immunological properties are destroyed and I am sure some of the vitamins also. Is it still superior to formula??? Probably. I have had a few mothers feed their babies refrozen human milk with no side effects. I can not recommend it, but just give you IMHO. Pat Bull, RN, IBCLC The Breastfeeding Connection/Medela Naperville, IL