Detecting Candida albicans in Human Milk Jimi Francis Morrill,1 Demosthenes Pappagianis,2 M. Jane Heinig,1 Bo Lönnerdal,1 and Kathryn G. Dewey1* Department of Nutrition,1 Department of Medical Microbiology and ImmunologyUniversity of California, Davis, Davis, California2 *Corresponding author. Mailing address: Department of Nutrition, University of California, One Shields Ave., Davis, CA 95616-8669. Phone: (530) 752-0851. Fax: (530) 752-3406. E-mail: [log in to unmask] Received June 3, 2002; Revised July 9, 2002; Accepted October 8, 2002. Abstract Procedures for diagnosis of mammary candidosis, including laboratory confirmation, are not well defined. Lactoferrin present in human milk can inhibit growth of Candida albicans, thereby limiting the ability to detect yeast infections. The inhibitory effect of various lactoferrin concentrations on the growth of C. albicans in whole human milk was studied. The addition of iron to the milk led to a two- to threefold increase in cell counts when milk contained 3.0 mg of lactoferrin/ml and markedly reduced the likelihood of false-negative culture results. This method may provide the necessary objective support needed for diagnosis of mammary candidosis. *********************************************** To temporarily stop your subscription: set lactnet nomail To start it again: set lactnet mail (or digest) To unsubscribe: unsubscribe lactnet All commands go to [log in to unmask] The LACTNET mailing list is powered by L-Soft's renowned LISTSERV(R) list management software together with L-Soft's LSMTP(R) mailer for lightning fast mail delivery. For more information, go to: http://www.lsoft.com/LISTSERV-powered.html