I don't know who posted theinfo at the bottom of hits post, but I passed it
on to my local nutritionist who called Nestle and found out the following:

Also, the website mention does not open for me.

Thanks,
Cindy


> Cindy,
>   I was curious enough to call Nestle.  The information you sent indicated
the
> producers of this bacteria were considering using it in several ways, but
have
> not yet.  The representative from Nestle told me she never heard of
> bifidobacteria in Carnation Good Start.  Check out their website
> (bestforbaby.com).

>
> "Cindy Curtis, RN, IBCLC" wrote:
>
> > Hi Ann
> >
> > Thought this may interest you ...... it did me!
> >
> > the reason the stools
> > > of this formula are more like breastmilk stools is because they use a
> > culture
> > > of bifidobacterium originally obtained from human fecal samples (of
course
> > > purified).  You may read all about it at the US Patent Office.  It's
> > patent #
> > > 5902743 called "Probiotic bifidobacterum strain" and filed in 1998.
> > > http://uspto.gov/patft/index.html
> > >
> > > "We took 196 human samples from a family (mother, infant, father,
> > sibling).
> > > Fecal samples (especially from the newborn infant and her sibling)
were
> > found
> > > to be the major source of bifidobacteria. Importantly, no
bifidobacteria
> > were
> > > found in 6 breast milk samples tested from the mother.
> > >
> > > A Bifidobacterium strain was found in oral and nasal samples taken
from
> > the
> > > infant immediately after birth, but not from these same sources when
> > sampled
> > > later on the same day. In contrast, no Bifidobacterium strain was
> > recovered
> > > from 11 fecal and 6 vaginal samples within the first 37 hours, but a
> > specific
> > > Bifidobacterium strain was recovered from infant fecal samples
obtained
> > from
> > > .gtoreq.38 hours to 1 year after birth. Bifidobacteria were detected
in
> > all
> > > 11 infant samples obtained at 6 months, but only in 4 out of the 13
> > samples
> > > obtained at 12 months.
> > >
> > > Genomic fingerprinting via pulsed field gel electrophoresis (PFGE) was
> > > conducted on intact genomic DNA from 16 Bifidobacterium strains from
the
> > > newborn female obtained from 6 positive samples (2 each from mouth,
nose,
> > and
> > > anus) taken immediately after birth and 1 fecal isolate from the
second
> > day
> > > (38 h). All 17 isolates recovered from the 7 positive samples
displayed
> > > essentially the same restriction fragment genomic fingerprints.
> > Essentially
> > > the same fingerprint elements were also observed in all 6
Bifidobacterium
> > > strains recovered from 2 fecal samples obtained from the mother at the
> > time
> > > of birth. Maternal feces (not breast milk) was therefore the source of
the
> > > Bifidobacterium found in the neonate. Since the breast milk itself did
not
> > > have the bacteria, breast milk appears to be a prebiotic, not a
probiotic.
> > >
> > > Bifidobacterium strains otherwise obtained from the infant within 1
month,
> > at
> > > 6 months and up to 3 years after delivery were also analyzed. Isolates
> > > recovered from the infant samples taken from delivery to 1 month
belonged
> > to
> > > a single clonal type. However, isolates recovered from samples
obtained at
> > 6
> > > months after birth contained three clonal types. Clonal type A was the
> > > predominant group observed in isolates from positive samples from
delivery
> > to
> > > 1 month. It was also present in bifidobacteria at six months, but was
not
> > the
> > > predominant group at 6 months.
> > >
> > > Between 1 month and 6 months the infant was fed solid foods and
received
> > some
> > > antibiotics. Thus, the different diet and/or medicine is suspected to
have
> > > caused subtle changes in the flora that were only discoverable via the
> > > conduct of PFGE.
> > >
> > > In addition to 6 isolates recovered from 2 fecal samples taken from
the
> > > mother at the time of birth, isolates from 3 fecal samples obtained
within
> > 1
> > > month after delivery also displayed the same or closely related
genomic
> > > fingerprints to that which predominated in the breast fed child. In
> > contrast,
> > > 5 isolates from one vaginal sample and another 2 fecal samples taken
from
> > the
> > > mother at the same period showed distinct genomic fingerprints. Also,
> > > isolates from the mother's fecal is samples taken at 6 and 12 months
> > > displayed significantly different fingerprints.
> > >
> > > These data indicated that a particular clone of bifidobacterium
> > predominated
> > > in this mother at parturition, and, thereafter, became much less
> > prevalent.
> > > Isolation of this Bifidobacterium clone from a healthy infant
throughout
> > the
> > > first 12 months is indicative of the safe nature of this bacterium in
> > vivo.
> > > Its predominance when the infant is youngest (and thus most
vulnerable)
> > > suggests its importance.
> > >
> > > We therefore chose this strain for the ATCC deposit referred to above,
and
> > > for use as a probiotic. The genomic fingerprint of this strain did not
> > match
> > > the genomic fingerprint of any of the known Bifidobacterium strains
that
> > we
> > > tested.
> > >
> > > Isolation
> > >
> > > As noted above, we obtained samples from a newborn infant (e.g.
feces). We
> > > cultured the sample overnight at 37.degree. C. in Reinforced
Clostridal
> > > medium, plus 0.5% propionic acid and 0.05% cysteine-HCl (pH 5.0)(RC5
> > medium)
> > > under anaerobic conditions. We then streaked portions therefrom onto
RC5
> > agar
> > > plates, and then incubated the plates 2-3 days at 37.degree. C. under
> > > anaerobic conditions.
> > >
> > > We then selected milky white, large colonies for further
characterization
> > and
> > > placed them in Brain Heart Infusion (BHI) broth supplemented with 0.5%
> > yeast
> > > extract, 0.5% dextrose and 0.05% cysteine-HCl. A representative colony
was
> > > selected and submitted for the previously mentioned ATCC deposit.
> > >
> > > EXAMPLES
> > >
> > > A preferred beverage of the present invention is infant formula such
as
> > > Carnation Good Start (Nestle Nutrition Division; Glendale, Calif.)
that
> > has
> > > been inoculated with the bacterium. The bacterium can also be
introduced
> > into
> > > various other beverages suitable for humans, especially infants and
> > toddlers.
> > > Examples could include, but not be limited to, fluid milks such as
Nutrish
> > > A/B produced by Mayfield Dairy Farms (Athens, Tenn.), fermented milks
such
> > as
> > > kefir produced by Lifeway Foods (Skokie, Ill.), and/or yogurts such as
> > > Mil--Mil produced by Yakult (Japan). Other bifid-amended beverages
could
> > > include fruit juices and/or sports drinks.
> > >
> > > Preferred food items that can be inoculated with the bacterium include
> > > dairy-based products such as natural cheese, cottage cheese, and ice
> > cream.
> > > Fruits and vegetables targeted for infants/toddlers, such as apple
sauce
> > or
> > > strained peas and carrots (Gerber Products Company; Fremont, Mich.)
are
> > also
> > > suitable for inoculation. Both infant cereals such as rice-or
oat-based
> > > cereals (Gerber) and adult cereals such as Musilix may also be
suitable
> > for
> > > this invention. In addition to foods targeted for human consumption,
> > animal
> > > feeds may also be suitable for inoculation. It may also be of benefit
to
> > > directly inoculate newborn humans or animals with a solution
containing
> > the
> > > preferred strain and to continue to dose such individuals with this
strain
> > at
> > > regular intervals throughout weaning and during periods of stress,
> > diarrhea,
> > > and transit.
> > >
> > >              ***********************************************
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>
>

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