Part 4 was rejected last night because it caused me to exceed my 3-per-day
limit. I will send Part 4 tomorrow. It is Appendix A Letter to Participants.
Pat Gima
Appendix A- Letter to Participant
Dear Participant,
First of all let me thank you very much for taking part in this study. Your
willingness to participate may shed light on the question at hand “Is it
safe to store breastmilk in the refrigerator after partial infant
feeding?”. This paperwork includes an explanation of your role in the
study, instructions regarding your participation, a questionnaire and a
consent form. You should also have received a “cooler kit”. This kit
contains a thermometer, two clean 8 ounce bottles with lids, an ice pack
and a carrying case. Use of these materials is explained below.
Please read all instructions before participating in the study. Completely
fill out the attached questionnaire and consent form. Review your “cooler
kit” contents, and place the enclosed ice pack in your home freezer.
On the day of the experiment, please bring with you: Completed paperwork,
baby (a little hungry), and 6-8 ounces of expressed milk. I have a large
variety of clean nipples. They include orthodontic, standard, latex and
silicon. If your baby uses a Platex nurser, please call me, because I am
limited on these supplies, but I can arrange for their availability.
Instructions for expression: Please express 6 - 8 ounces of breastmilk 12 -
36 hours before arriving. (That would be after 9 PM on Friday, but before 9
PM on Saturday.) Please wash hands for one minute in hot soapy water before
expressing. Express in your normal manner, and note method on
questionnaire. Use enclosed bottles for expression. “Double pumpers” can
use both bottles provided. Just combine the milk into one bottle before
storing in the refrigerator. Single pumpers can just use one bottle.
Please do whatever needed to get 6 - 8 ounces of breastmilk. This would
include pumping both breasts, or multiple pumping sessions within the 12-36
hour window. Note time and date of collection, and store the milk in your
refrigerator until Sunday morning. I have enclosed a thermometer (° C),
because I would like you to measure the temperature of your refrigerator.
Just write down the temperature on the enclosed questionnaire. While in
transit to Concordia, please place milk into cooler with ice pack.
Please arrive at Concordia University on Sunday, March 8th at 9 AM. Meet in
Luther Building, Room 316. (See attached map) You can park in the west
parking lot.
What to expect on Sunday: When you and your baby arrive, we will place the
eight ounce bottle of expressed breastmilk into a warm water bath for 5-10
minutes, or until 98° F. While waiting, baby will be given two sterile
swabs for saliva collection. Once heated, the bottle will be shaken and
split between two sterile four ounce bottles. Clean nipples will be placed
on both bottles. One bottle will be fed to baby for one minute, or until
one ounce is consumed. The other bottle will be used as a control. Both
bottles will be capped and refrigerated. Bacterial analysis will be
performed over the next 4 days.
Thank you in advance for your participation.
Sincerely,
Rachel R. Brusseau
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