http://www.blackwell-synergy.com/links/doi/10.1046%2Fj.1365-2184.36.s.1.7.x
Cell Proliferation
Volume 36 Issue s1 Page 73 - October 2003
doi:10.1046/j.1365-2184.36.s.1.7.x
Evidence of progenitor cells of glandular and myoepithelial cell lineages in
the human adult female breast epithelium: a new progenitor (adult stem) cell
concept
Werner Boecker and Horst Buerger
Abstract. Although experimental data clearly confirm the existence of
self-renewing mammary stem cells, the characteristics of such progenitor
cells have never been satisfactorily defined. Using a double
immunofluorescence technique for simultaneous detection of the basal
cytokeratin 5, the glandular cytokeratins 8/18 and the myoepithelial
differentiation marker smooth muscle actin (SMA), we were able to
demonstrate the presence of CK5+ cells in human adult breast epithelium.
These cells have the potential to differentiate to either glandular
(CK8/18+) or myoepithelial cells (SMA+) through intermediary cells (CK5+ and
CK8/18+ or SMA+). We therefore proceeded on the assumption that the CK5+
cells are phenotypically and behaviourally progenitor (committed adult stem)
cells of human breast epithelium. Furthermore, we furnish evidence that most
of these progenitor cells are located in the luminal epithelium of the
ductal lobular tree. Based on data obtained in extensive analyses of
proliferative breast disease lesions, we have come to regard usual ductal
hyperplasia as a progenitor cell-derived lesion, whereas most breast cancers
seem to evolve from differentiated glandular cells. Double
immunofluorescence experiments provide a new tool to characterize
phenotypically progenitor (adult stem) cells and their progenies. This model
has been shown to be of great value for a better understanding not only of
normal tissue regeneration but also of proliferative breast disease.
Furthermore, this model provides a new tool for unravelling further the
regulatory mechanisms that govern normal and pathological cell growth.
-----Original Message-----
From: Kocher, Polly K. [mailto:[log in to unmask]]
Sent: Friday, September 30, 2005 1:38 PM
To: [log in to unmask]
Subject: stem cells
The medical school in this community is all excited about stem cell
research using umbilical cord blood. I casually mentioned breastmilk
several times, and when I got a response they asked if stem cells in
breastmilk was my hypothesis or proven. Oh proven of course....then,
they did searches and found no such information. Believe me these
people would research stem cells in breastmilk, just as eagerly as they
do with the cord blood, (and probably save the world.) But, they cannot
find anything that backs my statement. Nor can I now, using the
Hospital Medical Librarian using all her magic tricks. My information
is gleaned from LactNet which makes several comments to human milk stem
cells totiary? value-meaning very pure/basic/totally available??? I'm
not sure. . Nothing I can use. Does anyone have evidence that stem
cells are present in breastmilk? ASAP?
Thanks!
Polly Kocher, IBCLC
OSF Breastfeeding Resource Center
People become really quite remarkable when they start thinking that they
can do things. When they believe in themselves, they have the first
secret to success.
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