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Date: | Mon, 14 Mar 2005 22:35:38 EST |
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FYI Chris
Eur J Endocrinol. 1995 Nov;133(5):613-7.
Usefulness of recombinant human prolactin for treatment of poor puerperal
lactation in a rat model.
Yoneda N, Irahara M, Saito S, Uemura H, Aono T.
Department of Obstetrics and Gynecology, School of Medicine, University of
Tokushima, Japan.
Recombinant human prolactin (r-hPRL) was produced by a line of murine C127
cells transfected with human PRL gene. To assess the biological efficacy of
r-hPRL in vivo, we studied its influence on milk secretion using a rat model in
which lactation was reduced by bromocriptine treatment. Puerperal rats were
injected daily for 9 days after delivery with bromocriptine or bromocriptine plus
r-hPRL, and lactational performance was assessed by weighing the pups. The
concentrations of rat and human PRL in rat serum were measured by specific
radioimmunoassays and the mammary glands were examined on postpartum day 10. Daily
injection of bromocriptine (0.1 mg/rat) significantly reduced the endogenous
level of rat PRL and impaired the weight gain of the pups. Administration of
r-hPRL increased the serum level of human PRL. Daily injections of r-hPRL (50
micrograms/rat, twice a day) restored lactational performance and significantly
increased the weight of the pups. The detrimental effect of bromocriptine on
the mammary glands, assessed by both weight and histological appearance, was
reversed by administration of r-hPRL. These results demonstrate that r-hPRL is
biologically active in vivo and replacement therapy of r-hPRL is effective in
improving the lactational performance in bromocriptine-treated rats, and also
that r-hPRL may be useful for the treatment of women with poor lactation.
PMID: 7581993 [PubMed - indexed for MEDLINE]
Christine Betzold NP IBCLC MSN
www.theBFclinic.com
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