Subject: | |
From: | |
Reply To: | |
Date: | Wed, 14 Jun 2000 08:16:08 EDT |
Content-Type: | text/plain |
Parts/Attachments: |
|
|
U.S. Class 800/235
Patent Number 5739407
Issue Date 1998 04 14
Assignee Symbicom aktiebolag
Inventor(s) Bergstrom, Sven
Hernell, Olle
Lonnerdal, Bo
Hjalmarsson, Karin
Hanson, Lennart
Tornell, Jan
Stromqvigt, Mats
Title Human .beta.-casein, process for producing it and use
thereof
Abstract The present invention relates to a DNA sequence encoding the
human milk protein .beta.-casein or an analogue or variant
thereof which has either the calcium binding activity of
human .beta.-casein, or opioid activity, or angiotensin
converting enzyme (ACE) inhibitory activity, or a
combination of any two or three of these activities. The DNA
sequence may optionally contain one or more intron sequences
and permissive RNA splice signals. The DNA sequence is used
in the production of recombinant human .beta.-casein,
advantageously by means of production in transgenic
non-human mammals such as bovine species. In one embodiment,
the DNA sequence is inserted into a milk protein gene of a
mammal such as a whey acidic protein (WAP) gene. The main
use of the recombinant human .beta.-casein is as a
constituent of infant formulae. It is contemplated that the
recombinant human .beta.-casein provides a substantial
improvement of the nutritional and biological value of the
formulae in that a closer similarity to human milk is
obtained.
Exmp. Claim 4
Ex Claim text A transgenic mouse whose germ cells and somatic cells
contain a DNA construct which comprises at least one human
beta casein intron sequence and a promoter of a milk protein
gene other than a casein gene, and a sequence which encodes
a polypeptide having at least 85% sequence identity with SEQ
ID NO: 2 or which hybridizes under stringent conditions with
SEQ ID NO: 1, as a result of chromosomal incorporation of
the construct into the mouse genome, or into the genome of
an ancestor of said mouse, so that the DNA construct is
expressed in a mammary gland of the transgenic mouse.
***********************************************
The LACTNET mailing list is powered by L-Soft's renowned
LISTSERV(R) list management software together with L-Soft's LSMTP(TM)
mailer for lightning fast mail delivery. For more information, go to:
http://www.lsoft.com/LISTSERV-powered.html
|
|
|