Suggestions to thread of where to put apistan Two deeps: I would put one strip per frame of bees as the label reads. Most of the year this coincides with where the brood is and there are more mites (80%) in the brood than on adults. As the new adults (bees) emerge the pregnant female mites are exposed to Apistan, hopefully before they can scurry into another cell that is ready to be capped. If there is no brood then all the mites are on the adults and strips should be placed among the bees, whether they are up or down. One deep: Put one strip per five frames where the bees are. If the bees are spread evenly throughout all frames then spacing between frame 3 and 4, and between frames 7 and 8 is fine, but if all the bees and brood are clustered to one side then strips should be evenly spaced in the cluster. Live long and pollinate, John John A. Skinner 218 Ellington Hall Extension Apiculturist University of Tennessee [log in to unmask] Knoxville, TN 37901 (423)974-7138