Dear All I'm not trying to be pedantic but as far as I understand it sterliisation is the killing of ALL living material, whereas disinfection is killing, removing or inactivating such organisms. So strerilisation of hive parts may well need require autoclaving. In an article in 1992 Prof Len Heath commented that AFB is relatively resistant to heat as it has large spores, nonetheless he suggests that there is some evidence that combs from infected colonies may be disinfected by being melted down and made into foundation. He was not advocating this and it would probably involve an element of risk. Many years ago EB Wedmore gave the following: Destruction of disease germs, AFB 12 minutes in water at 100C EFB 10 minutes in water at 65C He wrote this in 1932. Since the temperature required to melt wax for foundation is only 65C this is quite a wide divergence. Since hive parts have for many years been disinfected by scorching, is it not likely that a thorough steaming would prove effective at controlling AFB and EFB. Prof Heath also suggests that immersion for 5 - 10 minutes in Caustic Soda would leave hive parts infection free. Best wishes