For larger apiaries, I'd do an entire apiary sample from the entrances, then follow the protocol on my website for processing large samples. This will give you the most meaningful infection number, since you will likely be treating by yard, not by individual colony. Randy is right that we will be treating for the yard. But I prefer to take a 10% sample of the yard and analize each colony separatelly, if more than 50% of the samples are infected treat, if more than 30% is heavilly infected treat. -- Juanse Barros J. APIZUR S.A. Carrera 695 Gorbea - CHILE +56-45-271693 08-3613310 http://apiaraucania.blogspot.com/ [log in to unmask] *********************************************** The BEE-L mailing list is powered by L-Soft's renowned LISTSERV(R) list management software. For more information, go to: http://www.lsoft.com/LISTSERV-powered.html Access BEE-L directly at: http://community.lsoft.com/scripts/wa-LSOFTDONATIONS.exe?A0=BEE-L