Antonio Nanetti (AN) at Istituto Nazionale di Apicoltura in Bologna, Italy reported from a large number of trials on use of formic acid, oxalic acid (both spraying and trickling) and aerosol use of oxalic acid (with poor results).

Also tested has been Apilife VAR where specific attention has been given to the variability in amount of active ingredient in the product, which calls for better standadization of the treatment.

Copper gluconate has also been tested against Varroa without good results, nor has aerosol use of thyme (1%) and sage (0.5%) given satisfactory effects on the mite in field tests.