Greetings!
Holly-B Apiary
P.O.Box 26
Wells,Maine 04090-0026
www.cybertours.com/~midnitebee
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>Hi fellow Beehavers,
>    I have seen several posts on the use of apistan for maximum mite
>kill and to stop resistance to apistan,  you must use two strips per
>colony.
Stop resistence to apistan?..I do not believe you meant to say that, at
least not in that context....
I place apistan between the 3-4th frame and the 6-7th frame.
 
All colonies that winter in the north have at least two deeps on
>for the brood chambers.
you are running two deeps? why? There is a fellow in Buckfield that runs a
600 hive operation running with a single deep in the winter...
I live in the seacoast of Maine and I realize the temp's are quite different
Downeast.
I run one deep during the winter months with NO problem.
>My question is where would I put the apistan strips in the upper chamber
>or lower chamber?
for me?..i USE APISTAN STRIPS in the top hive w/grease
patties..reason?..bees move UP not down,.
>but I rotate the two brood chambers at least 3 times before the honey
>flow starts.
and you plan to have a 1000 hives...hope you have a strong back!
I rotate my brood chambers once per year.
Herb(Midnitebee)