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Subject:	Re: DWV test results for Newfoundland bee stock
From:	Peter Armitage <[log in to unmask]>
Reply To:	Informed Discussion of Beekeeping Issues and Bee Biology <[log in to unmask]>
Date:	Sat, 21 Oct 2017 19:30:40 -0400
Content-Type:	text/plain
Parts/Attachments:	text/plain (7 lines)

I had lunch yesterday with a member of our beek association research committee who is a medical researcher familiar with PCR methodologies.  He wondered about the PCR primers used in the Shutler, et al. paper for DWV diagnosis and suggested they could have had false positives for DWV because they amplified “junk.”  This stuff is over my head.  Anyone have a comment on these DWV primers? See Table 1 here http://nlbeekeeping.ca/data/documents/shutler_etal_2014.pdf 

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