"My conclusion is that a good rule of thumb estimation would be to use the
simple calculation that for every million spores per bee, you will see
about 5 spores in the field of view at 400x, when the bee sample is diluted
at the rate of 1ml water per bee. Thus, if one were to see, say, 15 spores
in a field, that would indicate an infection level of 3 x 106 spores per
bee."

This is with the dilution method. What about the squish method? Would you
use the Topolska and Hartwig method to count the spores? Or does it make no
difference?

Karen T-K

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