SUMMARY

*Nosema ceranae* is a widespread honeybee parasite, considered to be one of
the pathogens involved in the colony losses phenomenon. To date, little is
known about its intraspecific genetic variability. The few studies on *N.
ceranae* variation have focused on the subunits of ribosomal DNA, which are
not ideal for this purpose and have limited resolution. Here we
characterized three single copy loci (*Actin, Hsp70* and *RPB1*) in three *N.
ceranae*isolates from Hungary and Hawaii. Our results provide evidence of
unexpectedly high levels of intraspecific polymorphism, the coexistence of
a wide variety of haplotypes within each bee colony, and the occurrence of
genetic recombination in *RPB1*. Most haplotypes are not shared across
isolates and derive from a few frequent haplotypes by a reduced number of
singletons (mutations that appear usually just once in the sample), which
suggest that they have a fairly recent origin. Overall, our data indicate
that this pathogen has experienced a recent population expansion. The
presence of multiple haplotypes within individual isolates could be
explained by the existence of different strains of *N. ceranae* infecting
honeybee colonies in the field which complicates, and must not be
overlooked, further analysis of host–parasite interactions.

---------- Forwarded message ----------
From: Mariano Higes Pascual <[log in to unmask]>
Date: 2013/11/19
Subject:
To:


  *http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=9073257&fulltextType=RA&fileId=S0031182013001790
<http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=9073257&fulltextType=RA&fileId=S0031182013001790>*



*Regards*



*Mariano Higes**, DVM, PhD*

*Centro Apícola de Castilla-La Mancha*

*Laboratorio de Patología*

*Consejería de Agricultura *

*San Martín s/n. *

*19180 Marchamalo. Spain*

¡NUEVO TELÉFONO!: 949885014

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