The Swienty rep at the EAS conference in New Jersey stated that the CO2 knocks out the varroa as well as the bees, allowing them to be dislodged by shaking into count area.. Disposal of the live mites needs consideration. No information given on any adjustments due to not a true sample(as in a sugar roll), other than the sample being 200 bees. I have not tested due to failure to get mine back from beekeeper who was supposed to review. Sales rep info is !st hand info *********************************************** The BEE-L mailing list is powered by L-Soft's renowned LISTSERV(R) list management software. For more information, go to: http://www.lsoft.com/LISTSERV-powered.html