Ari asked: >>I have myself lots of quees that have been in hives for 2 treatments and can see no effects. Also have not seen anything hinting this in our data for 220 hives for 4 yeras. Even overdose or double tratments in fall have not given damage to the queen. Only kills a lot of bees during the winter. I only read about it somewhere. I treated 3 hives and believe to have applied too much of the [right concentration] solution. My observation was that the clusters coming out of the winter were smaller than usual. One queen disappeared, one queen stopped laying after about a month, the third queen laid up only 3 frames and her bees started supercedure. These had been all young queens raised in the previous July/August. There could have been other factors but I assumed the decline was due to my applying the full dose to each bee space occupied by bees. I later came to realize the dose should be directly proportional to the amount of bees in each bee space. >>For some reason that no one knows the bees can not take 2 treatments in fall. You mention double treatments have not damaged the queens in your experience. Are they 'tougher' than the worker bees? >>Spring treatment must be done immideately after cleansing flight for the colonies to be with only minimal of brood. Here in the north bees don't fly out at all for 4 - 6 months. A back-up treatment in the spring is always a great option. I have not done it. It's August, the bees have recovered, and I don't see varroa in drone brood or on the bees. I'll check varroa drops in September/October. Regards, Waldemar :::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::: -- Visit www.honeybeeworld.com/BEE-L for rules, FAQ and other info --- ::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::