Check the hive to see if there are any eggs.  If  there are any, you have
laying workers.  If not, you don t have laying workers yet.  Give them a
frame containing eggs and unsealed brood to deter laying workers.  The danger
here is that they will raise a queen from  freshly hatched larva.  You may
wish to let them do that if they will.
 
If you do have laying workers you have a real problem.  The following works
most of the time for me:
Make up a 2 or 3 nuc form another hive, using the new queen, and put it on
top of the queenless one above a double screen.  When the queen is accepted,
set the nuc off to the side and carry the old hive some distance away .  Take
all the frames out of the old hive and lean them up against something .
 Shake all bees off the hive body and bottom board and return  these to the
original location.  Next. Shake all bees off the frames ant put the frames
back in the hive.  Then, put the nuc back on top without the double screen.
 Most of the laying workers have never been  out of the hive and will get
lost in the grass.
When the new queen is accepted reverse the hive bodies so that the queen is
in the bottom brood chamber.   She should already be laying at this point and
will build up quickly
 
Frank Humphrey