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Subject:
From:
Renaud Lavend'Homme <[log in to unmask]>
Reply To:
Informed Discussion of Beekeeping Issues and Bee Biology <[log in to unmask]>
Date:
Sat, 6 Jan 2018 16:51:30 +0000
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Dear Randy,

> Interesting that trehalose is insect blood sugar (ours is glucose).
> I've just never seen it mentioned outside of that context.
and
> Funny how the food industry can invisibly start using something without the
> public being aware.

Trehalose is also used for queen insemination in the diluant or for  
semen centrifugation purpose. it is described in those 2 articles:

http://www.theriojournal.com/article/S0093-691X(11)00455-9/fulltext
http://www.bioone.org/doi/abs/10.1603%2FEC13159

some explanations why they have used trehalose:
"Trehalose was included in experiment two because it is known to  
stabilize biomembranes reviewed in [36], and serves as a natural  
cryoprotectant in numerous insects [reviewed in 37]. It is also  
present at low concentrations in honey bee seminal plasma [38].  
Trehalose, being the main blood sugar of insects and specifically  
transported across membranes, likely leads to an increase of metabolic  
activity. This is also suggested by the fact that it can lead to the  
restoration of motility in immotile honey bee semen [41]."

I tested this BSS buffer last year for my inseminations (single drone  
insemination and some multi drone also) and it worked very well, but I  
can't say if it is better (or worst) than my current buffer (hyes  
buffer)...I also used this buffer to homogenize semen (following the  
protocol of the article).

regards

renaud (Belgium)



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