I don't know who posted theinfo at the bottom of hits post, but I passed it on to my local nutritionist who called Nestle and found out the following: Also, the website mention does not open for me. Thanks, Cindy > Cindy, > I was curious enough to call Nestle. The information you sent indicated the > producers of this bacteria were considering using it in several ways, but have > not yet. The representative from Nestle told me she never heard of > bifidobacteria in Carnation Good Start. Check out their website > (bestforbaby.com). > > "Cindy Curtis, RN, IBCLC" wrote: > > > Hi Ann > > > > Thought this may interest you ...... it did me! > > > > the reason the stools > > > of this formula are more like breastmilk stools is because they use a > > culture > > > of bifidobacterium originally obtained from human fecal samples (of course > > > purified). You may read all about it at the US Patent Office. It's > > patent # > > > 5902743 called "Probiotic bifidobacterum strain" and filed in 1998. > > > http://uspto.gov/patft/index.html > > > > > > "We took 196 human samples from a family (mother, infant, father, > > sibling). > > > Fecal samples (especially from the newborn infant and her sibling) were > > found > > > to be the major source of bifidobacteria. Importantly, no bifidobacteria > > were > > > found in 6 breast milk samples tested from the mother. > > > > > > A Bifidobacterium strain was found in oral and nasal samples taken from > > the > > > infant immediately after birth, but not from these same sources when > > sampled > > > later on the same day. In contrast, no Bifidobacterium strain was > > recovered > > > from 11 fecal and 6 vaginal samples within the first 37 hours, but a > > specific > > > Bifidobacterium strain was recovered from infant fecal samples obtained > > from > > > .gtoreq.38 hours to 1 year after birth. Bifidobacteria were detected in > > all > > > 11 infant samples obtained at 6 months, but only in 4 out of the 13 > > samples > > > obtained at 12 months. > > > > > > Genomic fingerprinting via pulsed field gel electrophoresis (PFGE) was > > > conducted on intact genomic DNA from 16 Bifidobacterium strains from the > > > newborn female obtained from 6 positive samples (2 each from mouth, nose, > > and > > > anus) taken immediately after birth and 1 fecal isolate from the second > > day > > > (38 h). All 17 isolates recovered from the 7 positive samples displayed > > > essentially the same restriction fragment genomic fingerprints. > > Essentially > > > the same fingerprint elements were also observed in all 6 Bifidobacterium > > > strains recovered from 2 fecal samples obtained from the mother at the > > time > > > of birth. Maternal feces (not breast milk) was therefore the source of the > > > Bifidobacterium found in the neonate. Since the breast milk itself did not > > > have the bacteria, breast milk appears to be a prebiotic, not a probiotic. > > > > > > Bifidobacterium strains otherwise obtained from the infant within 1 month, > > at > > > 6 months and up to 3 years after delivery were also analyzed. Isolates > > > recovered from the infant samples taken from delivery to 1 month belonged > > to > > > a single clonal type. However, isolates recovered from samples obtained at > > 6 > > > months after birth contained three clonal types. Clonal type A was the > > > predominant group observed in isolates from positive samples from delivery > > to > > > 1 month. It was also present in bifidobacteria at six months, but was not > > the > > > predominant group at 6 months. > > > > > > Between 1 month and 6 months the infant was fed solid foods and received > > some > > > antibiotics. Thus, the different diet and/or medicine is suspected to have > > > caused subtle changes in the flora that were only discoverable via the > > > conduct of PFGE. > > > > > > In addition to 6 isolates recovered from 2 fecal samples taken from the > > > mother at the time of birth, isolates from 3 fecal samples obtained within > > 1 > > > month after delivery also displayed the same or closely related genomic > > > fingerprints to that which predominated in the breast fed child. In > > contrast, > > > 5 isolates from one vaginal sample and another 2 fecal samples taken from > > the > > > mother at the same period showed distinct genomic fingerprints. Also, > > > isolates from the mother's fecal is samples taken at 6 and 12 months > > > displayed significantly different fingerprints. > > > > > > These data indicated that a particular clone of bifidobacterium > > predominated > > > in this mother at parturition, and, thereafter, became much less > > prevalent. > > > Isolation of this Bifidobacterium clone from a healthy infant throughout > > the > > > first 12 months is indicative of the safe nature of this bacterium in > > vivo. > > > Its predominance when the infant is youngest (and thus most vulnerable) > > > suggests its importance. > > > > > > We therefore chose this strain for the ATCC deposit referred to above, and > > > for use as a probiotic. The genomic fingerprint of this strain did not > > match > > > the genomic fingerprint of any of the known Bifidobacterium strains that > > we > > > tested. > > > > > > Isolation > > > > > > As noted above, we obtained samples from a newborn infant (e.g. feces). We > > > cultured the sample overnight at 37.degree. C. in Reinforced Clostridal > > > medium, plus 0.5% propionic acid and 0.05% cysteine-HCl (pH 5.0)(RC5 > > medium) > > > under anaerobic conditions. We then streaked portions therefrom onto RC5 > > agar > > > plates, and then incubated the plates 2-3 days at 37.degree. C. under > > > anaerobic conditions. > > > > > > We then selected milky white, large colonies for further characterization > > and > > > placed them in Brain Heart Infusion (BHI) broth supplemented with 0.5% > > yeast > > > extract, 0.5% dextrose and 0.05% cysteine-HCl. A representative colony was > > > selected and submitted for the previously mentioned ATCC deposit. > > > > > > EXAMPLES > > > > > > A preferred beverage of the present invention is infant formula such as > > > Carnation Good Start (Nestle Nutrition Division; Glendale, Calif.) that > > has > > > been inoculated with the bacterium. The bacterium can also be introduced > > into > > > various other beverages suitable for humans, especially infants and > > toddlers. > > > Examples could include, but not be limited to, fluid milks such as Nutrish > > > A/B produced by Mayfield Dairy Farms (Athens, Tenn.), fermented milks such > > as > > > kefir produced by Lifeway Foods (Skokie, Ill.), and/or yogurts such as > > > Mil--Mil produced by Yakult (Japan). Other bifid-amended beverages could > > > include fruit juices and/or sports drinks. > > > > > > Preferred food items that can be inoculated with the bacterium include > > > dairy-based products such as natural cheese, cottage cheese, and ice > > cream. > > > Fruits and vegetables targeted for infants/toddlers, such as apple sauce > > or > > > strained peas and carrots (Gerber Products Company; Fremont, Mich.) are > > also > > > suitable for inoculation. Both infant cereals such as rice-or oat-based > > > cereals (Gerber) and adult cereals such as Musilix may also be suitable > > for > > > this invention. In addition to foods targeted for human consumption, > > animal > > > feeds may also be suitable for inoculation. It may also be of benefit to > > > directly inoculate newborn humans or animals with a solution containing > > the > > > preferred strain and to continue to dose such individuals with this strain > > at > > > regular intervals throughout weaning and during periods of stress, > > diarrhea, > > > and transit. > > > > > > *********************************************** > > > The LACTNET mailing list is powered by L-Soft's renowned > > > LISTSERV(R) list management software together with L-Soft's LSMTP(TM) > > > mailer for lightning fast mail delivery. 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