Hi
We are practicing hemolymph analisis (like a Blood test) for some years now.
We built a special kit to help the collection : a microcapillary is inserted between two tergites of the abdomen, near the bee heart and the hemolymph is coming by capillarity. then we blow the hemolymph (about 1-5 microliters) in a coated tube avoiding melanization. We apply then, for a single individual, a mass spectrometry method we name Maldi Bee-Typing, wich gives a profile of the haemolymph's peptides (600-18 000 Da). We can see the major peptides of bee immunity (Abaecin, Apidaecin, Defensin, hymenoptaecin) and some other molecules of interest. With another spectrometry method (ESI-LC-MS/MS) we can get a list of proteins, but we need to pool 5 hemolymphs.
At this time this method is mainly used to discriminate experimental conditions in research projects, and is very accurate in that : we can discriminate easily very tiny effects, as pesticides, when other methods do not see any impact.
We are now in a second phase, deciphering the impact of various stressors on bee hemolymph profile (pathogens, pesticides, starvation, heat shocks, etc). This part is ongoing, but the idea is to determine as you suggest,- if one bee is "healthy" or not.- if the immunity is stimulated- what type of stressors the bee may be confronted to- if the bee has the capacity to resist to the stressor (group of stressors)
Thanks for all the Bee-L contributers.
Michel (from France)
Le mercredi 20 mars 2024 à 10:15:44 UTC+1, Gustav Palan <[log in to unmask]> a écrit :
I have a question. Is there such a thing as bee hemolymph analysis? Is there a comprehensible laboratory exercise that teaches step by step the collection of bee hemolymph and the analysis of its basic components? Then, as a result, a comparison table with values of normal and outside the range of a healthy individual?
https://en.wikipedia.org/wiki/Hemolymph
Gustav Palan
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